Konjugasi pada Bakteri

Summary: Bacterial Conjugation

Bacterial conjugation is the transfer of genetic material from a donor bacterium to a recipient through direct cell-to-cell contact.

Main Steps

• Donor and recipient: The donor is an F⁺ cell, which contains an F plasmid; the recipient is an F⁻ cell, which lacks it.
• Cell contact: The donor uses an F pilus to attach to a receptor on the recipient cell and establish a connection.
• Plasmid preparation: An endonuclease cuts one strand of the plasmid at its origin of transfer.
• DNA transfer: The cut plasmid strand is transferred into the recipient.
• Strand completion: Each cell synthesizes a complementary strand, restoring a double-stranded plasmid.
• Outcome: The recipient acquires the F plasmid and becomes F⁺, so both cells are F⁺.

Speaker or Source

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