Summary of Agarose Gel Electrophoresis

The video explains the process of agarose gel Electrophoresis, a technique used to separate DNA fragments based on their size. Here are the key scientific concepts and procedural steps presented:

Scientific Concepts:

• Agarose Gel Electrophoresis: A method for separating DNA fragments using an electric current.
• DNA Charge: DNA molecules are negatively charged, causing them to migrate towards the positive electrode when an electric current is applied.
• Electrophoresis: The movement of charged particles through a medium under the influence of an electric field.

Procedure for Agarose Gel Electrophoresis:

1. Setup:
   • Align the agarose gel in the mini-sub cell with wells closest to the negative electrode.
   • Ensure the gel is placed in the gel chamber with electrodes positioned correctly.
2. Preparation:
   • Add Electrophoresis running buffer to the reservoirs at each end of the gel chamber, ensuring the wells are covered by at least 2 mm of buffer.
3. Loading Samples:
   • Use an adjustable micropipette to obtain DNA samples.
   • Load samples into the wells in the correct order, keeping the pipette tip perpendicular to the wells to avoid puncturing them.
   • Avoid movement of the gel chamber after loading to prevent spilling samples.
4. Running the Gel:
   • Place the lid on the gel chamber and connect the electrodes to the power supply (matching colors: black to black, red to red).
   • Set the power supply to the appropriate constant voltage and start the run.
   • Observe the migration of DNA samples from the wells towards the positive electrode, indicated by bubbles forming at the electrodes.

Researchers or Sources Featured:

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