Summary of Tools of Science: Testable Questions

Summary of "Tools of Science: testable questions"

This video explains the importance of testable questions in scientific research, using the example of marine microbiologists Kim and Kay studying how light affects the interaction between a phytoplankton species (Emiliania huxleyi) and the virus that infects it.


Main Ideas and Concepts


Methodology: Refining a Broad Question into a Testable Question

  1. Start with a broad curiosity:
    How does light affect host-virus interactions?
  2. Make it Specific:
    Change to: Does light affect host-virus interactions? (Yes/No question)
    Then refine to: Does light at different wavelengths and intensities affect host-virus interactions?
  3. Ensure it is Measurable:
    • Measure light intensity and wavelength.
    • Measure infected vs. non-infected cells.
    • Narrow down light intensities that do not harm the host but affect the virus.
  4. Check Achievability:
    • Confirm availability of equipment (adjustable lights, flow cytometer, sample cultures).
    • Confirm ability to grow organisms in the lab.
    • Confirm time available for experiments.
  5. Confirm Relevance:
    Focus on wavelengths and intensities of light actually present in the ocean environment.
  6. Add Temporal Component:
    Define the timeframe of interest (e.g., first 24-48 hours of infection).

Final Testable Question

"Does light at the wavelengths present in the ocean and intensities that do not affect the host affect the virus in the first 24 to 48 hours of infection?"


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